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United States Department of Agriculture

Agricultural Research Service

Title: Chicken Anti-Conoid Monoclonal Antibody Identifies An Common Epitope Which Is Present on Motile Stages of Eimeria, Neospora and Toxoplasma.

Authors
item Sasai, K - DEPT.VET.SCI. JAPAN
item Lillehoj, Hyun
item Hemphill, A - INST.PARASIT.SWITZERLAND
item Matsuda, H - HIROSHIMA UNIV. JAPAN
item Hanioka, Y - DEPT.VET.SCI. JAPAN
item Fukata, T - DEPT.VET.SCI. JAPAN
item Baba, E - DEPT.VET.SCI. JAPAN
item Arakawa, Akira - DEPT.VET.SCI. JAPAN

Submitted to: Journal of Parasitology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 2, 1998
Publication Date: N/A

Interpretive Summary: Avian coccidiosis is caused by several different species of intracellular protozoan parasites. Coccidiosis is primarily controlled by prophylactic medication. However, due to an increasing drug resistance of Coccidian parasites, development of non-drug control strategies is needed. In order to develop recombinant coccidia vaccine, ARS scientists developed a chicken nmonoclonal antibody which identifies a conserved apical complex antigen involved in parasite invasion of host cells. This antibody identifies an epitope on conoid and conserved across all six Eimeria species and two related apicomplexans. This antibody will be used to identify potential vaccine antigens for coccidiosis.

Technical Abstract: The chicken monoclonal antibody (mAb) 6D12-G10, raised against Eimeria acervulina sporozoites, has previously been shown to recognize the conoid of E. sporozoites and inhibit sporozoite invasion of lymphocytes in vitro. In this study, we examined the cross-reactivity of mAb 6D12-G10 with other motile stage of Eimeria, sporozoites from 6 different species of Eimeria and 2 other related apicomplexans. In indirect immunofluorescent assay, the mAb 6D12-G10 also reacted with merozoites from E. acervulina and identified a 21kDa merozoite protein in Western blots. By confocal laser scanning microscopy, the conoid of sporozoites from six different avian Eimeria species (E. brunetti, E. maxima, E. mitis, E. necatrix, E. praecox and E. tenella) were reactive with 6D12-G10 mAb. Furthermore, the 6D12-G10 mAb also showed cross-reactivity with motile stages of two closely related apicomplexans including Neospora and Toxoplasma. These results indicate that the mAb 6D12-G10 identifies a conserved epitope on the conoid which is important in host cell invasion by the apicomplexan parasites.

Last Modified: 10/22/2014