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United States Department of Agriculture

Agricultural Research Service

Title: Nutritional Bioavailability of Se in Broccoli

Authors
item FINLEY, JOHN
item Davis, Cindy
item Feng, Yi - UNIV OF NORTH DAKOTA

Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: April 17, 1998
Publication Date: N/A

Technical Abstract: Studies were conducted comparing the bioavailability of selenium (Se) in broccoli to other sources. Study 1: Rats (96 weanling males) were fed a torula yeast based diet with no added Se or 0.1 mg Se/kg as selenate (controls) for six weeks. Selenium deficient animals were then repleted (63 d) with Se adequate diets that contained 0.1 mg Se/kg supplied as selenate, selenite, selenomethionine (SeMet), or high-Se broccoli (hydroponically grown; 28 mg Se/kg dry). Absorption of Se from high-Se broccoli was lower than from other sources. Selenite, selenate and SeMet were similar in restoring most indices of Se status; broccoli was much less effective. However, Se from broccoli was as effective as other forms in restoring kidney and plasma Se. Study 2: Ten young men were fed a test meal containing stable Se in the form of selenate or incorporated into hydroponically grown broccoli. Plasma was chromatographed on Sephadex gel and fractions were analyzed for stable Se. Although less total isotope from broccoli was found in the plasma, both forms had a similar distribution of isotope among three plasma proteins. Findings from the rat study suggest that Se from broccoli is not as effective as selenite, selenate and SeMet in restoring most tissue Se concentrations and GSH-Px activities; however tissues behave differently. Chromatographic data from the human study do not show a clear difference between Se from broccoli and other forms of Se in the distribution of Se among plasma selenoproteins.

Last Modified: 9/29/2014
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