Author
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Johnson, William |
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LOZANO, AMY - 5450-20-00 |
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Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only Publication Acceptance Date: 4/18/1998 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: A previous study demonstrated that low copper (Cu) content in maternal diets diminished the postnatal accretion of protein kinase C (PKC) alpha, beta, and gamma isoforms in neonatal rat brain. Because of diminished accretion, brains in neonates of dams that consumed diets containing 1 and 2 ug Cu/g contained less PKC alpha, beta, and gamma at age 21 days than brains in neonates of dams that consumed diet containing 6 ug Cu/g. The present study examines whether diminished accretion alters the isoform content in regions of the neonatal brain. Three groups of dams consumed diets containing 1, 2, or 6 ug Cu/g (Cu1, Cu2, and Cu6, respectively) for a period beginning 3 weeks before conception and ending when the neonates were 21 days old. PKC isoform content was determined from immunoblots of high-speed supernatant fractions prepared from brain regions of the 21 day old neonates. Neonates of dams consuming Cu1 and Cu2 had lower contents of PKCbeta in the striatum, hypothalamus, hippocampus and cerebral cortex and PKCgamma in the striatum, hypothalamus, and cerebellum compared to neonates of dams fed Cu6 (P< 0.1, one-tailed Dunnett's contrasts). PKCalpha distribution was not affected by maternal Cu intake. These findings indicate that maternal Cu deficiency may influence postnatal brain development by altering the regional distribution of PKC isoforms. |
