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United States Department of Agriculture

Agricultural Research Service

Title: The Influence of Wheat Dehydration-Induced Proteins on the Function of Turkey Spermatozoa after 24 Hour in Vitro Storage

Authors
item Donoghue, Ann
item Walker Simmons, M - ARS, USDA, PULLMAN, WA

Submitted to: Journal Of Poultry Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 23, 1998
Publication Date: N/A

Interpretive Summary: The object of this study was to determine if dehydration-induced proteins from desiccation-tolerant wheat seeds could protect turkey sperm and improve survival and function after liquid storage. A partially-purified protein extract was isolated from dry, mature wheat embryos and added to semen diluent. Hens were inseminated with either fresh or semen stored 24 hours at refrigerator temperature and fertility and hatchability data collected. The addition of wheat protein extract to semen stored 24 hours improved fertility and hatchability of eggs over semen stored in diluent alone or supplemented with BSA. The dehydration-induced proteins isolated from wheat embryos are capable of protecting turkey sperm during in vitro storage.

Technical Abstract: Dehydration-induced proteins and other osmo-protectants enable plants to survive dehydration stress. The object of this study was to determine if dehydration- induced proteins from desiccation-tolerant wheat seeds could protect turkey sperm and improve survival and function after liquid storage. A partially-purified protein extract was isolated from dry, mature wheat embryos and added to semen diluent. Hens were inseminated wit either fresh or semen stored 24 h at 5C and fertility and hatchability data collected. The addition of 10 percent wheat protein extract to semen stored 24 h at 5C improved fertility and hatchability of eggs over semen stored in diluent alone or supplemented with 10 percent BSA. The dehydration- induced proteins isolated from wheat embryos are capable of protecting turkey sperm during in vitro storage and could potentially improve long term storage.

Last Modified: 7/24/2014
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