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United States Department of Agriculture

Agricultural Research Service

Title: Species-Specific Pcr Markers for Sugarcane and Related Taxa

item Pan, Yong-Bao
item Burner, David

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: January 18, 1998
Publication Date: N/A

Technical Abstract: The objective of this study was to develop species-specific PCR markers for F1 progeny selection in sugarcane breeding. Generic primers P1 and P2 primed DNA products from the 5S ribosomal DNA spacer were obtained from elite sugarcane varieties and several related species including Saccharum officinarum, S. spontaneum, Erianthus spp., three cytotypes (2n=30,60, and 90) of S. giganteum, Miscanthus sinensis, maize, and sorghum. The PCR products exhibited some length polymorphisms and Southern blot experiments indicated that PCR products from various taxa did not always cross hybridize. These PCR products were cloned into a plasmid vector and sequenced. We will discuss variations among these taxa and the prospects for developing species-specific PCR markers to screen for hybrids among inter-taxa crosses.

Last Modified: 4/22/2015
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