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Title: DETERMINATION OF COVERAGE AND REGIONAL RATES OF RECOMBINATION FOR THE PORCINE LINKAGE MAP BY FLUORESCENT IN SITU HYBRIDIZATION OF GENETIC MARKERS

Author
item Rohrer, Gary
item LOPEZ-CORRALES, NESTOR - ROSLIN INST., UK

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 1/1/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: We physically assigned genetic markers at 30 cM intervals throughout the genome to be able to determine genomic coverage and estimate regional rates of recombination for the porcine genome. This was accomplished by isolating YAC or cosmid genomic clones containing mapped microsatellite markers and then physically assigning the clones via fluorescent in situ hybridization (FISH). To date, 198 genetic markers have been mapped which were physically assigned by FISH. These results indicate that the coverage of the genetic maps for 14 of the 19 chromosomes are virtually complete and most likely the coverage of SSC2 is also complete. There are four chromosomal regions void of markers, most of which are pericentric regions on acrocentric chromosomes. Regions void of markers are SSC11q1.7, SSC14q1.1-1.2, SSC16q1.1 and SSC18q1.1-1.2. The centromere has been localized to an interval less than 10 cM for SSC1, SSC2,SSC3, SSC6, SSC7, SSC8 and SSC9 while the only submetacentric chromosomes with the pericentric interval greater than 20 cM were SSC11 and SSC12. In general, rate of recombination per unit of DNA was greatest in regions near the telomeres and least near the centromeres. Also, rate of recombination was higher in small chromosomes. Two additional regions with extremely low rates of recombination were detected at SSC1q2.1-2.8 and SSQ13q2.4-4.1. Three additional regions with high rates of recombination were SSC5p1.2-1.5, SSC6p1.3-1.4 and SSC12p1.4-1.5. The well anchored genetic map will be beneficial for positional cloning and microdissection. It will also provide an estimate of the amount of DNA (number of YAC clones needed to produce a contig) spanning adjacent genetic markers.