ARS | Publication request: ISOLATION OF BRUCELLA ABORTUS STRAIN RB51 FROM VACCINATED BISON (BISON BISON)

Submitted to: Brucellosis Research National Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: October 8, 1997
Publication Date: N/A

Technical Abstract: Recovery of Brucella abortus RB51 was attempted from tissues of vaccinated bison on tryptose agar with 5% bovine serum (TSA), modified Kuzdas and Morse (MKM), and two new selective media, B. abortus RB51 (BRB) and brucellae malachite green medium (BMG). Ability of each medium to control contaminant growth was determined. Female bison (n=20) were vaccinated with approximately 1.4 X 10*10 CFU of RB51 at three to six months of age. At 14, 18, 22, 26, and 30 weeks post inoculation, four vaccinates were sacrificed and tissues collected in an attempt to recover RB51. Tissues were minced, inoculated onto the media, and incubated for seven days at 37C in 5% CO2. During the course of this study, RB51 was recovered from 42 samples on TSA, 19 samples on MKM, 24 samples on BRB, and 38 samples on BMG medium. RB51 was recovered on only one medium from 21 samples with the remaining media culture negative (9 on TSA, 3 on BRB, and 9 on BMG). Growth of contaminating bacteria prevented detection of RB51 from 41 samples on TSA, 24 on MKM, 3 on BRB, and 25 on BMG. Our data suggest that MKM medium is inferior to BRB and BMG for recovering RB51 from tissue samples and that BRB and BMG, when combined with TSA, enhance the ability to recover RB51 from tissue samples.