Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: November 12, 1997
Publication Date: N/A
Technical Abstract: To date, nine genes involved in the trichothecene biosynthetic pathway of F. sporotrichioides have been mapped within a 25kb gene cluster. Tri12, the most recently identified gene in the cluster, appears to encode a transport protein. Sequence analysis of this gene indicates there are 3 introns ranging in size from 48 to 59bp. The open reading frame of 1794bp specifies a protein of 598aa containing 12 predicted transmembrane segments. Comparison of the amino acid sequence to other known sequences indicates Tri12p is most closely related to the yeast multi-drug resistant protein, SGE1. Disruption of the Tri12 gene in Fusarium results in transformants that fail to accumulate either T-2 toxin or trichothecene pathway intermediates. These results suggest Tri12 is a transport protein and that it is required for trichothecene production.