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United States Department of Agriculture

Agricultural Research Service

Title: Rapid Communication: Identification of Two Microsatellite Loci Which Map to Porcine Chromosome 3.

item Rohrer, Gary
item Stone, Roger
item Troyer, Deryl - KANSAS STATE UNIV.

Submitted to: Journal of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: January 12, 1998
Publication Date: N/A

Technical Abstract: Source of clones: Porcine genomic DNA library was screened for microsatellites as described by Stone et al. (1997). Primers were developed as described by Rohrer et al. (1994). Chromosome location. There were 164 informative meioses for KS1 and 166 informative meioses for KS2. Two-point linkage analyses were conducted with CRI-MAP 2.4 (Green et al., 1990) between these two markers and all markers present in the MARC genome database. The most significant linkages detected were with SW274 (LOD=45.46 and theta =.00) and SW717 (LOD=23.33 and theta=.04) for KS1 and KS2, respectively. Multipoint linkage analyses indicated that the most likely position for KS1 was at position 0.0 cM and 122.8 cM for KS2 on the map published by Rohrer et al. (1996). Figure 1 presents an ideogram of the updated linkage group. Comments. These two markers may be useful to scientists mapping QTL on porcine chromosome 3. Both markers were easy to score and had 100% heterozygosity in the parents of the reference population. The fact that KS2 only had three alleles but was 100% heterozygous gives weak evidence that breed specific alleles for this marker may exist. Presence of breed specific alleles is extremely useful in multibreed resource populations but precludes this markers utility to detect QTL within a breed. Furthermore, the location of KS2 was in a region of the SSC3 map which has a low marker density. KS1 displayed nine alleles indicating that it could be useful in any population.

Last Modified: 4/17/2014