|Mecey-Smith, Christy - USDA-ARS-USWCL, PHOENIX|
|Tomasi, Pernell - ARIZONA STATE UNIV|
Submitted to: Association for the Advancement of Industrial Crops Conference
Publication Type: Abstract Only
Publication Acceptance Date: February 5, 1997
Publication Date: N/A
Technical Abstract: Emasculation is one of the most costly and labor intensive components of hybrid seed production. The most efficient and effective substitute for emasculation is introduction of male sterility into elite germplasm. Therefore, a significant step toward commercialization of Lesquerella fendleri will be identifying genes and mechanisms responsible for genic and gene-cytoplamatic male sterility. We have initiated a genetic search with the objective of identifying molecular markers diagnostic to male sterility. A sample of 20 plants, composed of ten male sterile plants, and 10 male fertile plants are used for the initial screening. Two molecular markers systems: Random Amplified Polymorphic DNA (RAPD) and Amplified Fragment Length polymorphism (AFLP) are used in search for markers that identify chromosonal segments associated with male sterility. Primers amplifying DNA segments known to be associated with male-sterile cytoplasm in other Brassicaea are applied as well. An up- to-date report of potentially diagnostic markers, as well as a discussion of plausible male sterility mechanisms in L. fendleri will be presented.