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Title: MOLECULAR SYSTEMATICS OF LESQUERELLA AND PHYSARIA SPECIES

Author
item Kaufman, Benjamin
item Richards, Stacy
item Dierig, David

Submitted to: Association for the Advancement of Industrial Crops Conference
Publication Type: Abstract Only
Publication Acceptance Date: 2/5/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The systematics of the genus Lesquerella was largely deduced from morphological, physiological and cytological characters. We are reporting the progress in the first molecular systematic study of the genus. Fifty two species and accessions from the USDA-ARS Water Conservation Lab Lesquerella germplasm collection are included in the study. These samples represent species and populations distributed throughout the United States. Also included are 29 species and populations of Physaria, and Brassica nappus as an outgroup. A novel DNA isolation was developed and employed in isolating DNA. Each Operational Taxonomic Unit (OUT) is represented by DNA pooled from ten randomly chosen individuals. We have applied DNA marker analyses of Random Amplified Polymorphic DNA (RAPD) and Amplified Fragment Length polymorphism (AFLP). A phenetic interpretation of the data addressing the limits of the genus, the systematic placement of Physaria and the division between western- eastern species will be presented.