ARS | Publication request: REGENERATION SAMPLING AND GENETIC PROFILES OF RYEGRASS ACCESSIONS

Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: September 1, 1996
Publication Date: N/A

Technical Abstract: Minimizing genetic changes in accessions associated with regeneration is critical for germplasm maintenance. Original seeds from three annual ryegrass (Lolium multiflorum) accessions were grown under field conditions to develop balanced (an equal number of seeds per plant), spike (an equal number of spikes per plant) and bulk (seeds proportionally assembled according to yield per plant) regeneration samples. Analyses of eight isozymes were completed on each population. Balanced samples deviated the least from original samples in allelic frequencies, but geneti diversity--as measured by mean heterozygosity and allelic richness--was not greatly different from the original populations in any sample, even after a second regeneration. The results suggest that key components of diversity could be maintained through bulk or spike samples, and at far less cost than developing balanced samples.