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Title: CHARACTERIZATION OF THE 1B/1R TRANSLOCATION IN WINTER WHEAT CULTIVARS BY PAGE, SDS PAGE, AND HPCE

Author
item WAGA, JACEK - PLANT BREEDING & ACCLIMT
item Bietz, Jerold

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 9/23/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The 1B/1R wheat/rye translocation increases wheat#s yield and disease resistance, but can decrease its breadmaking quality. Gel electrophoresis reveals a block of rye secalin proteins coded by translocated 1R genes in these lines. In previous studies, we used reversed-phase high-performance liquid chromatography and high-performance capillary electrophoresis (HPCE) to analyze blocks of wheat gliadins coded by specific alleles. Such blocks can provide markers of quality that are useful to breeders. In these earlier studies, we found some variation in protein amounts within gliadin blocks that may affect wheat quality. We therefore used HPCE to further characterize proteins of genotypes having the 1B/1R translocation. Wheat varieties were first analyzed by gel electrophoresis to select individual kernels with known gliadin and glutenin alleles. Kernels containing the Gli B1l block were then analyzed by HPCE to examine quantitative variation in proteins coded by the Gli B1l block that may be related to improved technological properties of 1B/1R genotypes. The use of such variants as markers during breeding may help overcome problems associated with presence of translocated rye genes in many wheat varieties.