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United States Department of Agriculture

Agricultural Research Service

Title: A Competitive Elisa for the Detection and Quantification of Fmrfamide-Like Peptides in Free-Living and Parasitic Nematodes

Authors
item MASLER, EDWARD
item Kovaleva, Elena - DEPT ZOOLOGY, UNIV OF MD
item Kingan, Timothy

Submitted to: Society of Nematology Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: July 9, 1997
Publication Date: N/A

Technical Abstract: A peptide family with a characteristic RFamide C-terminus ("FMRFamides") and possessing myoactive properties has been reported in both free-living and animal-parasitic nematodes (Review: Brownlee et al. 1996, Parasitology Today 12:343-351). Primary structures reported thus far probably represent only a fraction of those which are present in these animals. Immunoscreening has typically been done using radioimmunoassays (RIA). We have developed an enzyme-linked immunosorbent assay (ELISA), which detects 1fmol FMRFamide, as an additional biochemical tool. The assay has been used to monitor FMRFamide- like activity in extracts of Panagrellus redivivus (mixed developmental stages) and in subsequent chromatographic fractions. To facilitate characterization of new peptide sequences, we are developing an isolation protocol for FMRFamide-like peptides from P. redivivus and other free-living and plant- and animal-parasitic nematodes using the ELISA screen.

Last Modified: 9/10/2014
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