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Title: A COMPETITIVE ELISA FOR THE DETECTION AND QUANTIFICATION OF FMRFAMIDE-LIKE PEPTIDES IN FREE-LIVING AND PARASITIC NEMATODES

Author
item Masler, Edward
item KOVALEVA, ELENA - DEPT ZOOLOGY, UNIV OF MD
item Kingan, Timothy

Submitted to: Society of Nematology Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 7/9/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: A peptide family with a characteristic RFamide C-terminus ("FMRFamides") and possessing myoactive properties has been reported in both free-living and animal-parasitic nematodes (Review: Brownlee et al. 1996, Parasitology Today 12:343-351). Primary structures reported thus far probably represent only a fraction of those which are present in these animals. Immunoscreening has typically been done using radioimmunoassays (RIA). We have developed an enzyme-linked immunosorbent assay (ELISA), which detects 1fmol FMRFamide, as an additional biochemical tool. The assay has been used to monitor FMRFamide- like activity in extracts of Panagrellus redivivus (mixed developmental stages) and in subsequent chromatographic fractions. To facilitate characterization of new peptide sequences, we are developing an isolation protocol for FMRFamide-like peptides from P. redivivus and other free-living and plant- and animal-parasitic nematodes using the ELISA screen.