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United States Department of Agriculture

Agricultural Research Service

Title: A Recombinant Elisa Antigen Expressed in Baculovirus and Its Use in Serodiagnosis of Bovine Viral Diarrhea in Cattle Sera

item El-Kholy, Alaa - ARC, CAIRO, EGYPT
item Arab, Refaat - ARS, CAIRO, EGYPT
item Bolin, Steven
item Abou-Zeid, Abou-Zeid - ARS, CAIRO, EGYPT
item Hammam, H - ARS, CAIRO, EGYPT
item Ridpath, Julia

Submitted to: American Society for Microbiology
Publication Type: Abstract Only
Publication Acceptance Date: May 8, 1997
Publication Date: N/A

Technical Abstract: A recombinant baculovirus was generated that expresses the p14 and gp48 proteins of the NADL strain of bovine viral diarrhea virus (BVDVr1448) in insect cells (Sf9). The recombinant protein was antigenically authentic as shown by the western immunoblot, immunofluorescence, and immunoperoxidase staining in infected Sf9 cells. Crude lysate containing the recombinant protein (r1448) was used as a coating antigen in an indirect enzyme-linked immunosorbent assay (ELISA) for detection of antibody against BVDV. Th ELISA was compared to standard virus neutralization test (VNT) for detection of antibody against BVDV in cattle sera. Antibody titers determined by ELISA closely correlated with antibody titers determined by VNT (r=0.9303). The recombinant protein was an effective, sensitive and inexpensive ELISA antigen for detection of antibody against both genotypes of BVDV (1 & 2) in reference hyperimmune cattle sera. These findings indicate that the p14 and gp48 proteins of pestiviruses are useful for diagnostic tests and may have use as effective recombinant vaccines.

Last Modified: 4/21/2015
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