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United States Department of Agriculture

Agricultural Research Service

Title: Use of 1,25-Dihydroxyvitamin D3 As An Adjuvant Enhances Antibody Titers to J-5 Vaccine in the Cow

item Stabel, Judith
item Reinhardt, Timothy
item Goff, Jesse

Submitted to: International Veterinary Vaccines and Diagnostics Conference
Publication Type: Abstract Only
Publication Acceptance Date: July 27, 1997
Publication Date: N/A

Technical Abstract: The 1,25-dihydroxyvitamin D3 has been shown to be an immunomodulatory agent. Recent evidence in cattle and mice has suggested that 1,25-dihydroxyvitamin D3 may stimulate T-lymphocyte differentiation pathways responsible for humoral immunity (TH2). The 1,25-dihydroxyvitamin D3 was tested as an adjuvant for an Escherichia coli J5 vaccine. Ten mid-lactation cows received 2 ml of a commercial J5 vaccine and were revaccinated six weeks after the first vaccine administration. Five of these cows received 200 ug of 1,25-dihydroxyvitamin D3 in the primary vaccination and received an additional 200 ug of 1,25-dihydroxyvitamin D3 one week after the primary vaccination. No 1,25-dihydroxyvitamin D3 was administered with the week 6 vaccine booster. Milk IgM, IgG, and IgA antibodies to E. coli J5 were significantly increased in cows receiving 1,25-dihydroxyvitamin D3 plus E. coli J5 vaccine compared to cows which received only E. coli J5 vaccine. Serum IgG and IgG1 antibodies to E. col J5 also were significantly enhanced by 1,25-dihydroxyvitamin D3 treatment compared to cows receiving vaccine alone. In contrast, serum IgG2 titers tended to decline in cows receiving 1,25-dihydroxyvitamin D3. Taken together these data suggest that 1,25-dihydroxyvitamin D3 has potential usefulness in boosting humoral responses to some vaccines.

Last Modified: 4/22/2015