Submitted to: Society of Industrial Microbiology Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: April 17, 1997
Publication Date: N/A
Microbial biosensors were constructed using whole cells of Gluconobacter oxydans immobilized onto pH-sensitive field-effect transistors (FET). Glucose was measured over a range of 0.01 to 10 mM; xylose was measured from 0.1 to 100 mM, alone or in the presence of 0.1 mM glucose. Biosensors had a linear response range of approximately 0.07 to 1.0 mM for glucose, and 5.0 to 100 mM for xylose. For xylose in the presence of glucose, experimental responses agreed well with theoretical additive values within the range of 1.0 to 10.0 mM xylose. However, responses deviated greatly from theoretical values in the range of 10 to 100 mM xylose. Nevertheless, results suggest that it should be possible to employ pattern recognition analysis in conjunction with dilutions and/or a second sensor to simultaneously measure glucose and xylose.