|Wang, Z - CHINESE VISITING SCIENTIS|
|Irey, M - US SUGAR CORPORATION|
|Lockhart, B - UNIV OF MINNESOTA|
Submitted to: American Society of Sugar Cane Technologists
Publication Type: Abstract Only
Publication Acceptance Date: May 15, 1997
Publication Date: N/A
Technical Abstract: Sugarcane commercial cultivars and parental clones used in the Florida, Louisiana and Texas crossing programs at Canal Point were assayed for the presence of yellow leaf syndrome (YLS). The CP 95 Series clones for the cooperative breeding program that had been advanced to the Stage III were also assayed. The sugarcane yellow leaf virus was detected by a polymerase chain reaction (PCR) assay using YLS primers developed by M. S. Irey (US Sugar Corp.) and ELISA using antisera developed by B. E. Lockhart (Univ. of Minn.). Of the 46 CP parental and/or commercial clones used in Florida only six (CP 57-603, CP 82-1592, CP 89-1509, CP 92-1167, CP 92-1647, and CP 92-1684) were negative in both assays. Of the 71 parental clones used in the Louisiana and Texas breeding programs only four (CP 57-614, CP 92-624, HoCP 93-741, and TCP 91-3543) were negative in both the assays. Of the 136 CP-95 Series clones plus check cultivars in Stage II, 47.8% were positive using the PCR assay. The high incidence of YLS in the CP clones indicates resistance is either lacking or a low level. The lower incidence in the CP-95 Series clones may reflect a lack of opportunity of infection since the plants had been derived from true seed only two years previously. Although plants with the YLS symptoms usually assayed positive for sugarcane yellow leaf virus, a high number of plants that assayed positive for the virus had no symptoms. Yield losses in Florida have not been quantified.