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United States Department of Agriculture

Agricultural Research Service

Title: Restriction Endonuclease Analysis (Rea) of Pasteurella Multocida Associated with Hemorrhagic Septicemia - for the Fourth Int. Meet. on Bacterial Epidemiological Markers (Imbem), September 10-13, 1997, Denmark

item Rimler, Richard

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: June 25, 1997
Publication Date: N/A

Technical Abstract: Hemorrhagic septicemia (HS), an acute and highly fatal disease caused by Pasteurella multocida belonging to serogroup B or E, principally affects cattle and water buffalo. Occasionally, other animals such as swine and elephants are affected. P. multocida of serotypes B:2 and B:3,4 (225 strains isolated from animals in Europe, Asia, North America, and Africa) were examined by SDS-PAGE and REA. The objectives of this research were t determine diversity that may occur within serotypes and relate the findings to the epidemiology of HS. Little or no distinction was seen between protein patterns of strains within a particular serotype. Forty-two different DNA fingerprint profiles were recognized among 190 serotype B:2 strains using HhaI endonuclease. Twelve DNA fingerprint profiles were found among 35 serotype B:3,4 strains with the same enzyme. Further distinction could be made among some serotype B:2 or B:3,4 strains with HpaII endonuclease. For example, treatment with HpaII resulted in seven different DNA fingerprint profiles within 56 serotype B:2 strains that produced a profile designated HhaI 0018. Thus, a strain could be assigned a descriptive identification epithet (DIE) code such as B:2/HhaI 0018/HpaII 0006 or B:2/HhaI 0018/HpaII 0007. After REA, strains within the B:2 serotype isolated from outbreaks of HS in various animals of the same and different countries were compared. For example, among 64 B:2 strains from Sri Lanka, 24 fingerprint profiles were defined using HhaI endonuclease. Further, among 17 strains designated B:2/HhaI 0018, originating from animals such as cattle, buffalo, swine, and elephant, six HpaII profiles were found. Similar profiles were found also among isolates from swine and water buffalo from India.

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