Skip to main content
ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Ruminant Diseases and Immunology Research » Research » Publications at this Location » Publication #80927
Title: NEW FLOW CYTOMETRIC TECHNIQUES FOR THE ANALYSIS OF BLOOD FROM LARGE ANIMALS

Author
item Pesch, Bruce
item RAJARAMAN, VEENA - IOWA STATE UNIVERSITY
item Kehrli Jr, Marcus
item Harp, James

Submitted to: International Virtual Conference on Infectious Diseases of Animals
Publication Type: Abstract Only
Publication Acceptance Date: 4/20/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: A variety of newly developed flow cytometric techniques adapted for the bovine are described. Among these are: CD45 labeled/side scatter comparison for doing differentials in whole blood; apoptosis (programmed cell death) as determined using Annexin V-FITC; PKH2 (a green lipophilic membrane dye) determination of cell proliferation; and 3-color assays using PerCP and PE in conjunction with the Annexin and PKH2 techniques.