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United States Department of Agriculture

Agricultural Research Service

Title: New Flow Cytometric Techniques for the Analysis of Blood from Large Animals

Authors
item Pesch, Bruce
item Rajaraman, Veena - IOWA STATE UNIVERSITY
item Kehrli Jr, Marcus
item Harp, James

Submitted to: International Virtual Conference on Infectious Diseases of Animals
Publication Type: Abstract Only
Publication Acceptance Date: April 20, 1997
Publication Date: N/A

Technical Abstract: A variety of newly developed flow cytometric techniques adapted for the bovine are described. Among these are: CD45 labeled/side scatter comparison for doing differentials in whole blood; apoptosis (programmed cell death) as determined using Annexin V-FITC; PKH2 (a green lipophilic membrane dye) determination of cell proliferation; and 3-color assays using PerCP and PE in conjunction with the Annexin and PKH2 techniques.

Last Modified: 8/2/2014
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