Submitted to: American Society for Microbiology
Publication Type: Abstract Only
Publication Acceptance Date: May 7, 1997
Publication Date: N/A
Technical Abstract: Spirochete strain C1 induces diarrhea in chickens. Multilocus enzyme electrophoresis analysis and 16S rRNA sequence comparisons have indicated this strain is phylogenetically related to Serpulina species. These investigations were intended to further characterize this spirochete and establish its taxonomic identity. C1 cells formed weakly beta-hemolytic colonies on trypticase-soy agar plates containing 5% bovine blood. In BHIS broth (brain-heart infusion plus calf serum), C1 cells grew well (10e+9 cells/ml; 2-4 h pop. doubling time), hydrolyzed hippurate, and were negative for indole production. In HS broth (heart infusion plus calf serum plus glucose), under an O2-N2 (1:99) atmosphere, cells of the spirochete produced H2, CO2, acetate, butyrate, and ethanol. Glucosamine, N-acetyl glucosamine, glucose, glucuronic acid, maltose, and mannose were growth substrates for C1 cells in HS broth. Strain C1 DNA had a G+C content of 24.6 moles% and exhibited relative reassociation values of 24- 39% with DNAs from S. hyodysenteriae B78, S. murdochii 56-150, S. pilosicoli Wes-B, S. intermedia PWS/A, and S. innocens B256. These results indicate strain C1 has traits typical of the genus Serpulina and, based on DNA homology experiments, is a new Serpulina species.