ARS | Publication request: FORMATION AND PRODUCTION OF CHLAMYDOSPORES IN SOYBEAN SUDDEN DEATH SYNDROMECAUSING-ISOLATES OF FUSARIUM SOLANI

Submitted to: Mycological Society of America
Publication Type: Abstract Only
Publication Acceptance Date: June 5, 1997
Publication Date: N/A

Technical Abstract: Fusarium solani isolates that cause soybean sudden death syndrome (SDS) produced chlamydospores under defined conditions. Six isolates were examined for their potential to produce chlamydospores at different temperatures. Macroconidia of each isolate produced on plates of Bilay's medium were placed in sterile distilled water (pH 5.8) and incubated at 4,20,25 or 30 oC. Plates were examined weekly for chlamydospore production. Chlamydospores formed over time from either germinated macroconidia or hyphae, and at either the apical or basal end of a macroconidium. At 4 oC, macroconidia did not germinate in distilled water and all chlamydospores formed in the apical and basal cells of macroconidia. At 20 - 25 oC, which favored the germination of macroconidia, most chlamydospores formed from germ tubes and hyphae. However, at 30 oC, intercalary formation of chlamydospores, was most prevalent. More than one chlamydospore formed within a single macroconidium. The percentage of chlamydospores produced by macroconida significantly (P<0.003) differed by temperature. Production of chlamydospores varied among isolates. These results provide evidence that SDS-causing isolates of F. solani produce chlamydospores in distilled. water