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United States Department of Agriculture

Agricultural Research Service

Title: Evaluation of a Filter Bag System for Ndf, Adf, and Ivdmd Analysis for Use in Forage Breeding

Authors
item Vogel, Kenneth
item Pedersen, Jeffrey
item Masterson, Steven
item Toy, John

Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: July 2, 1998
Publication Date: N/A

Interpretive Summary: New laboratory methods for determining forage quality were evaluated for their utility in forage breeding programs. In the new methods, forage samples are sealed in filter bags and processed in a batch basis rather than on an individual basis as in the conventional analyses. The filter bag and conventional analyses systems were evaluated using smooth bromegrass, switchgrass, and forage sorghum samples. The results of the study indicate that the fiber bag analyses systems ranks forage samples from breeding programs in the same relative order as conventional analyses methods. The fiber bag method also has distinct advantages in terms of laboratory safety and labor and time management.

Technical Abstract: A filter bag analyses system for determining neutral and acid detergent fiber (NDF and ADF, respectively) and in vitro dry matter digestibility (IVDMD) were evaluated for use in forage breeding programs. In both filter bag systems, i.e. Ankom Fiber Analyzer and Ankom Rumen Fermenter systems, the forage samples are sealed in filter bags and the analyses are conducted on a batch basis rather than on an individual basis as in the conventional IVDMD and fiber analyses procedures. Smooth bromegrass (Bromus inermis Leyss.), switchgrass (Panicum virgatum L.), and forage sorghum (Sorghum bicolor (L.) Moench) samples were analyzed for NDF, ADF, and IVDMD using convention analyses and filter bag procedures. NDF and ADF values were 9% lower and IVDMD values were 7% higher using the filter bag system compared to conventional analyses. The method x entry interaction effects were usually not significant or were very small in comparison to method and entry effects. Spearman rank correlations were high and significant. These results indicate that the fiber bag analyses systems ranks forage samples from breeding programs in the same relative order as conventional analyses methods. The fiber bag method also has distinct advantages in terms of laboratory safety and labor and time management.

Last Modified: 11/22/2014