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United States Department of Agriculture

Agricultural Research Service

Title: Fermentation and Formulation of Dendryphion Penicillatum, a Potential Mycoherbicide of Papaver Somniferum

Authors
item Hebbar, Prakash - OICD
item Oneill, Nichole
item Bailey, Bryan
item Lumsden, Robert

Submitted to: National American Phytopathology Meetings
Publication Type: Abstract Only
Publication Acceptance Date: May 3, 1997
Publication Date: N/A

Technical Abstract: Ability of Dendryphion penicillatum, a potential mycoherbicide of Papaver somniferum, to grow and produce desiccation resistant microsclerotia was tested in shake culture flasks containing various concentrations (0.1, 1, 2.5, 5.0%) of V8 juice medium, Molasses yeast medium, potato dextrose medium, and water extracts of soya bean hull fiber, corn cob, bran, and cotton seed flour (Pharmamedia). Growth, conidia and microsclerotia formation in liquid cultures depended on the type and concentration of the growth substrate. Microsclerotia were formed readily in substrates with low utilizable carbon, such as aqueous extracts of Pharmamedia. Growth for 10 days in aqueous extracts of 1% Pharmamedia resulted in 10**4 microsclerotia ml**-1. In 2.5 l fermentors, with sparging, 104 ml**-1 of microsclerotia and conidia were produced after 14 days of growth. Viable counts from air dried biomass were 10**5 cfus g**-1 dry weight. Biomass obtained was formulated into a novel rice formulation. The shelf life at room temperature of the formulated D. penicillatum, ability to produce secondary propagules, and to infect poppy seedlings are being investigated.

Last Modified: 12/19/2014
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