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United States Department of Agriculture

Agricultural Research Service

Title: Effect of Degree and Duration of Heat Transmission of the High Plains Pathogen (Hhp) by Seed Vascular Puncture Inoculation (Vpi)

item Louie, Raymond

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: June 15, 1997
Publication Date: N/A

Technical Abstract: Initial transmission rates of HPP, a putative virus, to sweet corn seeds (Zea mays) var. Spirit using VPI were inconsistent (0-75%). Factors most likely affecting transmission rates were the degree and duration of heat during pre- and post-inoculation treatments. To test these possibilities, preinoculation soaking (PS) seeds in water at 11, 21, or 30 degrees C for 2 hr and postinoculation incubation temperatures (PIT) of 11, 21, or 30 deg C for 1 or 2 da (test 1) and PS seeds in water at 11, 21, or 30 deg C for 1, 2 or 4 hrs and a PIT of 30 deg C for 1 or 2 da (test 2) were studied. Each treatment of 50 inoculated kernels was replicated four or five times in a randomized block design. Infection rates of 2 da postinoculation incubation were significantly better (P greater than 0.01) than 1 da (ave. 48 vs 40% and 63 vs 51% for test 1 and 2, respectively). PIT at 30 deg C (test 1) also was significantly better than 11 or 21 deg C (64 vs 18 or 49%, respectively). Interactions of PIT x 1 or 2 da (test 1) and PS x 1 or 2 da (test 2) also were significant. PS treatments were not significant. Heat variables for VPI were optimized (64%) at 30 deg C for PS for 2 hr and PIT at 2 da.

Last Modified: 5/5/2015
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