Submitted to: American Veterinary Medical Association Abstract
Publication Type: Abstract Only
Publication Acceptance Date: April 18, 1997
Publication Date: N/A
Technical Abstract: Seventeen restriction endonucleases were evaluated for use in DNA fingerprinting of Riemerella anatipestifer. Digestion of chromosomal DNA with either HinfI or DdeI restriction endonuclease, followed by submarine electrophoresis in agarose and staining with ethidium bromide, resulted in DNA fingerprint profiles which could be easily distinguished. HinfI produced readable fingerprint patterns in the 2.7 to 20 kb range and was used to distinguish DNA fingerprint profiles. Among 89 strains of R. anatipestifer isolated from various avian species in the United States, the United Kingdom, Australia, Canada, Germany, and Israel, a total of 52 distinct DNA fingerprint profiles were found.