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United States Department of Agriculture

Agricultural Research Service

Title: 1,25-Dihydroxyvitamin D3 Inhibits Interferon-Gamma (Ifn-Gamma) Secretion Lectin-Stimulated Mononuclear Leukocytes from Dairy Cattle

item Ametaj, Burim - IOWA STATE UNIVERSITY
item Nonnecke, Brian
item Reinhardt, Timothy
item Beitz, Donald - IOWA STATE UNIVERSITY

Submitted to: International Virtual Conference on Infectious Diseases of Animals
Publication Type: Abstract Only
Publication Acceptance Date: April 20, 1997
Publication Date: N/A

Technical Abstract: We have shown that 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3], the most potent biological metabolite of vitamin D, modulates the proliferation and differentiation of bovine CD4+ and CD8+ PMNL in vitro. To further our understanding of this compound's effects on bovine PMNL function, the effect of 1,25-(OH)2D3 and two synthetic analogues, 22-26-F3-1,25-(OH)2D3 and 1,25,28-(OH)3D2, on IFN-gamma secretion by PMNL from ovalbumin (OVA)-sensitized adult dairy cattle were evaluated. Mononuclear leukocyte cultures were unstimulated or stimulated with pokeweed mitogen (5 and 10 mg/ml); OVA (16 and 32 mg/ml) or lipopolysaccharide (LPS, 10 mg/ml). Vitamin D concentrations in cultures were 0, 10**-10, 10**-9, 10**-8 moles/L. Culture supernatants were harvested at 24 and 48h and analyzed for IFN-gamma using an IFN-gamma-capture ELISA. Interferon-gamma secretion by resting and LPS-stimulated cultures was undetectable in control and vitamin D-supplemented cultures. 1,25-(OH)2D3, at 10**-9 and 10**-8 moles/L, inhibited (P<.05) secretion by PWM-stimulated cells; however, the synthetic analogue, 22-26-F3-1,25-(OH)2D3 at 10**-10 to 10**-8 moles/L inhibited (P<.001) IFN-gamma secretion by PWM-stimulated cells under all culture conditions.

Last Modified: 5/5/2015
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