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United States Department of Agriculture

Agricultural Research Service

Title: Molecular and Cellular Analysis of Beta-1,3-Glucanase Involvement in a Male-Sterile Soybean Mutant

Authors
item Wei, J - IOWA STATE UNIVERSITY
item Palmer, Reid
item Shoemaker, Randy
item Horner, H - IOWA STATE UNIVERSITY

Submitted to: American Society of Plant Physiologists Meeting
Publication Type: Abstract Only
Publication Acceptance Date: April 17, 1997
Publication Date: N/A

Technical Abstract: A large number of genes are involved in successful microsporogenesis in flowering plants. Analyses of soybean mutants have identified at least seven genetic loci that, when disrupted, yield arrested or developmentally abnormal male cells. Some of these mutations affect the early stages of microsporogenesis. In our Midwest Oil Seeds mutant, in vitro enzyme analyses showed no callase (beta-1,3-glucanase) activity in male-sterile mutant anthers at the tetrad stage of microsporogenesis. These results and microscopy studies suggest that male-sterility was caused by retention of the callose walls. Northern blots probed with beta-1,3-glucanase showed a reduction in callase mRNA in the male-sterile mutant. In situ hybridization studies showed that transcription, however did occur in the both normal and mutant tapeta just prior to the tetrad stage. Antibodies are being used to locate the site and time of beta-1,3-glucanase production nin the normal mutant anther tissues. Western blots are being conducted to determine whether translation is affected in this male-sterile mutant.

Last Modified: 12/18/2014
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