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United States Department of Agriculture

Agricultural Research Service

Title: Feasibility Studies for in Vitro Grafting and Chimera Formation among Lycopersicson Spp.

item Stephenson, Katherine - UNIVERSITY OF MARYLAND
item Stommel, John

Submitted to: Journal of American Society of Horticulture Science
Publication Type: Abstract Only
Publication Acceptance Date: June 1, 1997
Publication Date: N/A

Technical Abstract: A protocol to make in vitro graft unions among Lycopersicon spp. was developed and regenerates obtained from cultured graft unions evaluated for chimera formation. Young seedlings were preconditioned for four to six days in liquid half- strength Murashige and Skoog (MS) basal medium supplemented with 8.9 uM benzyladenine and 1.0 uM indole-3-butyric acid. Preconditioned seedlings exhibited increased biomass and enhanced graft union survival; in particular, cleft graft survival increased from 37% to 95% with seedling preconditioning. When graft unions among different genotypes were excised from apex-to-apex in vitro cleft grafts and plated on MS basal medium supplemented with 9.1 uM zeatin and 3.9 uM ancymidol, as many as 100 plantlets were regenerated from a single graft union. However, no chimeric regenerates were recovered, indicating that asymmetric responses to grafting may be a limiting factor to in vitro chimera formation.

Last Modified: 4/22/2015
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