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United States Department of Agriculture

Agricultural Research Service

Title: Humoral and Cell-Mediated Immune Response to Vaccinia Virus Vectors Expressing Glycoproteins Gb Or Gd of Pseudorabies Virus

Authors
item Brockmeier, Susan
item Wannemuehler, Michael - IOWA STATE UNIVERSITY
item Palmer, Mitchell
item Mengeling, William

Submitted to: International Virtual Conference on Infectious Diseases of Animals
Publication Type: Abstract Only
Publication Acceptance Date: April 1, 1997
Publication Date: N/A

Technical Abstract: The humoral and cell-mediated immune response to glycoproteins gB and gD of pseudorabies virus (PRV) delivered by recombinant vaccinia viruses was investigated. Pigs vaccinated with the recombinant gD vaccine responded with high virus neutralizing antibody titers approximating those of a group vaccinated with an attenuated PRV vaccine, while pigs vaccinated with the recombinant gB vaccine had an average virus neutralizing titer 16-fold lower. After vaccination, 5/5 pigs vaccinated with an attenuated PRV vaccine responded with lymphocyte proliferative responses to PRV antigen in vitro, while only 2/5 pigs receiving the gB recombinant and 1/5 pigs receiving the gD recombinant responded with a lymphocyte proliferative response to PRV antigen in vitro. One pig that was vaccinated with the gB recombinant, and none of the pigs given the gD recombinant, had a delayed-type hypersensitivity (DTH) response to PRV antigen. However, all 5 pigs vaccinated with the attenuated PRV vaccine responded with DTH responses to PRV, the gB recombinant, and the gD recombinant. The DTH response to PRV and the gB recombinant were of equal intensity and duration while the DTH response to the gD recombinant was not as large and resolved earlier. Thus, a cell-mediated immune response to gB and gD are apparently features of PRV infection; however, the study was unable to demonstrate consistent cell-mediated responses to gB or gD delivered through the recombinant vaccinia vector.

Last Modified: 7/28/2014
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