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Title: INDUCTION OF GLYCOPROTEIN B (GB)-SPECIFIC CYTOTOXIC T CELLS AFTER VACCINATION WITH RECOMBINANT FOWL POXVIRUS EXPRESSING GB

Authors
item Schat, K - CORNELL UNIVERSITY
item Omar, A - CORNELL UNIVERSITY
item Lee, Lucy
item Hunt, Henry

Submitted to: International Marek's Disease Symposium Abstracts and Proceedings
Publication Type: Proceedings
Publication Acceptance Date: September 7, 1996
Publication Date: N/A

Technical Abstract: Chickens vaccinated with recombinant fowl poxvirus expressing Marek's disease virus (MDV) glycoprotein B (rFPV-gB) were protected against challenge with MDV. Virus-neutralizing antibodies were detected in these chickens, but the presence of gB-specific cytotoxic T cells (CTL) was not examined. We investigated the possible role of CTLs in protective immunity yafter vaccination with rFPV-gB. Groups of P2a (B**19B**19) chickens were vaccinated with SB-1, rFPV-gB, or FPV or were left uninfected. CTLs were obtained from spleens between 5 and 14 days post inoculation (dpi) and used in chromium release assays against reticuloendotheliosis virus-transformed cell lines expressing MDV gB or pp38. CTLs specific for pp38 and gB were present in SB-1-vaccinated chickens between 5 and 14 dpi, while gB-specific CTL were present in rFPV-gB inoculated birds between 7 and 14 dpi. The rFPV-induced, gB-specific CTL were CD4-CD8**TCR alpha beta**+.

   
 
 
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