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United States Department of Agriculture

Agricultural Research Service

Title: Characterization and Geographical Distribution of a New Ilarvirus from Fragaria Chiloensis

Authors
item Spiegel, S - VOLCANI CTR,AG.RES.ORG.
item Martin, R - AG.CANADA RES.STA., B.C.
item Leggett, F - AG.CANADA RES.STA., B.C.
item Ter Borg, M - AG.CANADA RES. STA., B.C.
item Postman, Joseph

Submitted to: American Phytopathological Society
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 1, 1993
Publication Date: N/A

Interpretive Summary: Virus infected strawberry plants are often symptomless. When vegetative plant material is moved across national boundaries, there is a possibility of international movement of virus diseases. A previously unknown virus was found in strawberry plants that were brought to the US from Chile following a USDA funded plant collecting expedition. This paper describes the geographical distribution and the physical and biochemical characteristics of this virus, for which the name Fragaria Chiloensis Ilarvirus (FCIV) is proposed.

Technical Abstract: A previously undescribed virus, a new member of the ilarvirus group, was isolated from wild Fragaria chiloensis plants collected in Chile & imported into the US during 1990 & 1992. The virus, for which the name fragaria chiloensis ilarvirus (FCIV) is proposed, was detected during the postquarantine period in eight symptomless accessions collected in different locations in Chile. FCIV was transmitted mechanically to Chenopodium quinoa, C. amaranticolor, and Cucumis sativus but not to other herbaceous plants tested and was also transmitted through seeds collected from naturally infected F. Chiloensis plants. FCIV particles, purified from inoculated C. quinoa, were quasi-isometric with a diameter of 21.4 nm, & bacilliform particles to 54.5 nm in length, containing a single polypeptide with relative molecular mass (Mt) of 28,000 & 4 RNA molecules of 3,700, 2,700, 2,600, and 1,200 bases, respectively. A polyclonal & a monoclonal antiserum to FCIV were produced. FCIV was related serologically to the ilarviruses asparagus virus II and lilac ring mottle in indirect enzyme- linked immunosorbent but not in immunosorbent electron microscopy assays. Based on tests with F. chiloensis accessions collected in the wild in Chile and along the Pacific coast of the US and Canada, FCIV seems to be geographically limited to Chile.

Last Modified: 8/19/2014
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