Submitted to: Virus Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: May 3, 1997
Publication Date: N/A
Interpretive Summary: Viruses belonging to the pestivirus group are an important cause of economic loss to the pork and cattle industries worldwide. Three diseases are caused by pestiviruses; bovine viral diarrhea/mucosal disease in cattle, hog cholera in swine, and border disease virus in sheep. The genetic material of viruses that cause bovine viral diarrhea and hog cholera have been defined. Recently, we derived the first to complete genetic sequence border disease virus. The genetic sequence of this virus is clearly distinct from the viruses that cause bovine viral diarrhea virus and hog cholera. This information is of immediate benefit to APHIS and state veterinary diagnostic laboratories because it allows differentiation of the pestiviruses by DNA-based tests. There is a long-term benefit to producers and veterinary biologics manufacturers because this information is crucial for design of new and improved vaccines for pestiviruses.
Technical Abstract: The genus Pestivirus is composed of hog cholera virus (HCV), bovine viral diarrhea virus (BVDV), and border disease virus (BDV). Complete sequences have been published for HCV and the two genotypes of BVDV (BVDV1 and BVDV2). In this study the complete sequence of the border disease virus (BDV), BD31, was determined. BD31 was isolated from a lamb with hairy shaker syndrome and is the BDV type virus offered by ATCC (ATCC VR-996). The genome was 12,268 nucleotides long and had a single large open reading frame (ORF) beginning at nucleotide 357 and ending at nucleotide 12045. The sequence identity of the predicted amino acid sequence of BD31 and other published pestivirus sequences varied from 71% to 78%. Phylogenetic analysis of available complete genomic sequences segregated pestiviruses into two branches. One branch contained BD31 and HCV isolates while the other branch contained BVDV1 and BVDV2 isolates. Pestiviruses from the same branch were similar in the length of the 5 and 3 untranslated regions regions (UTR). When complete genomic sequences were compared among BD31, HCV, BVDV1 and BVDV2, the highest sequence identity was observed in the 5 UTR. Within the ORF, the highest sequence identity was observed in the genomic region coding for the nonstructural viral polypeptide p80.