Submitted to: Society of Industrial Microbiology Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: August 7, 1997
Publication Date: N/A
Technical Abstract: Oxygenated metabolites of unsaturated fatty acids play a variety of important roles in biological systems. We have begun investigating the production of value-added chemicals from vegetable oils and their component fatty acids, i.e. the production of 10-hydroxy and 10-keto saturated or unsaturated fatty acids; and a new dihydroxy unsaturated fatty acid which has antimicrobial activity. Recently, we discovered a novel compound, 12,13,17 trihydroxy 9(Z) octadecenoic acid, which was produced from linoleic acid by microbial transformation at 25% yield. The newly isolated microbial strain catalyzing this transformation was identified as Clavibacter sp. ALA2. The product was purified by high pressure liquid chromatography, and its structure was determined by 1H and 13C nuclear magnetic resonance, Fourier transform infrared, and mass spectroscopy. Structure of this compound resembles those "plant self defense substances". Other than extraction from plant materials and chemical synthesis, this is the first report on 12,13,17-trihydroxy unsaturated fatty acid and its production by microbial transformation.