Submitted to: American Society for Virology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: July 23, 1997
Publication Date: N/A
Technical Abstract: Phylogenetic analysis of complete genomic sequences segregate pestiviruses into two branches. Each branch can be further subdivided into two genotypes. One branch is made up of the hog cholera virus and "true" border disease virus genotypes. The other is composed of the bovine viral diarrhea virus 1 and 2 genotypes. Viruses from the two branches differed 5' UTR length and in the presence of repeated nucleotides at end of the 3' UTR. Highest sequence identity observed among pestiviruses was in the 5' UTR, where sequence identities ranged from 65% to 68% between viruses from different branches greater than or equal to 81% between viruses within the same genotype. Within the ORF, the highest sequence identity was observed in the region coding for the nonstructural viral polypeptide p80. Predicted amino acid sequence identity of the complete ORF varied from 71% to 73% between viruses from different branches to greater than or equal to 80% between viruses within the same genotype. Predicted gylcosylation site and the sequences flanking the predicted cleavage site between the p20 and p14 proteins were highly conserved. Sequences flanking other putative cleavage sites were more variable.