ARS | Publication request: Analysis of Saponins in Peanut Using Hplc and Fast Atom Bombardment Mass Spectrometry

Submitted to: Inform
Publication Type: Abstract Only
Publication Acceptance Date: January 1, 1997
Publication Date: N/A

Technical Abstract: An HPLC method has been developed to separate saponins found in peanut seed from other components. Soxhlet and accelerated extraction which utilizes solvent, increased temperature, and pressure were used to obtain an oil extract from peanut seed for saponin analysis. Soya-like saponins separated by HPLC were characterized both by HPLC/Fast Atom Bombardment (FAB) and static FAB in the positive and negative modes. A Zorbax xdb C8 column (2.1 mm x 150 mm) with a solvent system of methanol/hexane/0.05 ammonium acetate/glycerol (84/7.4/8/10.6, vol/vol) was used to separate peanut saponins from other components in the extract. Saponins were found in both the cotyledons and embryos of peanuts. The identity of Soyasaponin I was confirmed. Diagnostic ions and catonized ions characteristic of Soyasaponin III and A2 were present in the FAB mass spectral data. Some other unidentified minor saponin-like compounds were present in the extract. This technique provided a fingerprint composition of soya-like saponins found in peanuts.