Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 25, 1997
Publication Date: N/A
Interpretive Summary: We have demonstrated that genomic scans with microsatellite markers spaced at 20 cM intervals are powerful tools to identifying regions of the genome which contain the genes responsible for phenotypes controlled by a single locus, regardless of the mode of inheritance. By utilizing multiplexed PCR reactions, these scans can be performed at less than 50% of the cost when compared to amplifying each microsatellite separately. Other methods, such as genotyping extremes or an iterative procedure to eliminate regions of the genome, can be implemented to make QTL research even more economical.
One hundred fifty-four microsatellite markers were selected to be used in a genomic scan of the porcine genome. The selection criteria were ease of scoring, level of polymorphism, genetic location and ability to be genotyped in a multiplexed PCR reaction. The selected microsatellites were chosen to span the entire genome flanked by the porcine linkage map with intervals between adjacent markers of 15-20 cM where possible. The utility of this set of markers was demonstrated by linkage analyses with loci controlling blood plasma protein and red cell enzyme polymorphisms (n = 13), erythrocyte antigens (n = 15), the S blood group, coat color and ryanodine receptor from 174 backcross Meishan-White Composite pigs. These loci displayed various forms of inheritance. Significant two-point linkages (LOD > 3.0) were detected for each polymorphic marker. These results provide the first linkage assignments for PGM2 and EAF to SSC8; and AMY and EAI to SSC18. All of the remaining loci mapped to previously identified regions. The marker set should be useful in resource populations of various breed crosses since the number of alleles detected in a multi-breed reference population was one of the selection criteria.