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United States Department of Agriculture

Agricultural Research Service

Title: Examination of Extraintestinal Tissue Cysts of Isospora Belli

Authors
item Lindsay, David - AUBURN UNIVERSITY, AL
item Dubey, Jitender
item Toivio-Kinnucan, M - AUBURN UNIVERSITY, AL
item Michiels, J - UNIVERSIT DE NICE, FRANCE
item Blagburn, Byron - AUBURN UNIVERSITY, AL

Submitted to: Journal of Parasitology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: February 3, 1997
Publication Date: N/A

Interpretive Summary: Members of the genus Isospora are coccidian parasites, normally infecting the intestines of man and animals. They have a simple fecal/oral cycle. The parasites develop in the intestine and a resistant stage (oocyst) is excreted in feces. Scientists at the Beltsville Agricultural Research Center and Auburn University report extra intestinal infection of Isospora belli in a human patient. They describe the structure of the parasite in detail to help pathologists diagnose this infection. The results will be useful to human pathologists and parasitologists in general.

Technical Abstract: Relapse is common in immunocompetent and immunosuppressed humans infected with Isospora belli). Relapse is believe to be associated with the presence of extraintestinal stages. Histological, immunohistological, histochemical, and ultrastructural methods were used to examine extraintestinal tissue cysts of Isospora belli in an AIDS patient. Anti-sera made in rabbits to Isospora suis, Toxoplasma gondii, Hammondia hammondi, Sarcocystis neurona, Neospora caninum, and Caryospora bigenetica were tested against I. belli tissue cysts in the avidin-biotin peroxidase complex immunohistological (ABC) test. Most anti-sera reacted positively in the ABC test at dilutions of 1:100 but not at dilutions of 1:250. Some anti-sera to N. caninum and H. hammondia reacted positively at dilutions of 1 :1,000 in the ABC test. Most reactive anti-see stained the tissue cyst wall and not the enclosed zoite. Eight histochemical tests were examined and most were non-reactive with I. belli zoites or tissue cysts. Transmission electron microscopy revealed that the tissue cyst wall was composed of granular material and it was directly beneath the parasitophorous vacuole membrane. Zoites were in the center of the tissue cysts and surrounded by fibrillar material that appeared to originate from the zoite surface. Tubule-like structures were present in the granular tissue cyst wall and in the fibrillar material that surrounded the zoite. Zoites contained a crystalloid body.

Last Modified: 12/22/2014