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United States Department of Agriculture

Agricultural Research Service

Title: Section 5: DNA Fingerprinting of M. Bovis Isolates

Author
item Whipple, Diana

Submitted to: Assessing the Risks Associated with M Bovis in Michigan Free Ranging White
Publication Type: Other
Publication Acceptance Date: September 13, 1996
Publication Date: N/A

Technical Abstract: Several methods of DNA fingerprinting are used to detect genetic differences and similarities among Mycobacterium bovis isolates from various sources. These methods include restriction endonuclease analysis, restriction fragment length polymorphism (RFLP) analysis, and analysis of enterobacterial repetitive intergenic consensus sequences amplified by the polymerase chain reaction (ERIC-PCR). M. bovis isolates from free-ranging white-tailed deer in Michigan were analyzed by RFLP and ERIC-PCR. In addition, RFLP analysis was used for DNA fingerprinting an M. bovis isolate from a heifer in Wisconsin. The RFLP patterns for the 17 deer isolates and the heifer isolate were identical when DNA was digested with AluI and hybridized with pTBN12. Sixteen of the 17 deer isolates and the heifer isolate have a single copy of the insertion sequence IS6110. When DNA was digested with PvuII and hybridized with a probe that binds twice for every copy of IS6110, slight differences were observed in the RFLP patterns for of the 16 isolates with a single copy of IS6110. Results of ERIC-PCR indicate that all M. bovis isolates from the Michigan deer are identical. Results of DNA fingerprinting are being used by regulatory officials for epidemiological investigation of tuberculosis in Michigan and Wisconsin.

Last Modified: 10/20/2014
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