ARS | Publication request: MOLECULAR MARKERS ASSOCIATED WITH LOW PROTEIN IN BARLEY.

Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: October 30, 1996
Publication Date: N/A

Technical Abstract: Malting barley (Hordeum vulgare L.) cultivars inherently low in grain protein are needed by the malting and brewing industries. In environments favoring low protein, differentiating between lines that have low protein and those that do not is often difficult. Identification of molecular markers associated with low-protein factors would allow barley breeders to identify low-protein lines independent of environmental effects. F2:5 progeny of the cross ND5377 (low protein) by Azure (high protein) were used as sources of materials for protein and genomic DNA. F2:5 families from this cross were grown in the field at Fargo and Langdon in 1995. This study found two RAPD primers were associated with low protein. They explained 20 and 38% of the variation in protein of F2:5 families, respectively. The estimated linkage between low protein loci and the two RAPD-markers is 31 and 25 cM, respectively. Chi-square analysis showed the two markers to be linked and the linkage distance is 31 cM. Collectively, these RAPD-marker loci explain about 40% of the variation in protein of F2:5 families. Results suggest two low-protein loci are segregating in the cross evaluated. The chromosomal location of the loci is inconclusive.