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United States Department of Agriculture

Agricultural Research Service

Title: Sequence Diversity and Genotyping

Author
item Ridpath, Julia

Submitted to: Meeting Abstract
Publication Type: Proceedings
Publication Acceptance Date: June 25, 1996
Publication Date: N/A

Technical Abstract: The currently recognized species, of the pestivirus genus, are bovine viral diarrhea virus (BVDV), hog cholera virus (HCV), and border disease virus (BDV) of sheep. These viruses are associated with enteric disease, respiratory disease, reproductive failure, and immunosuppression in host animals. Segregation of pestiviruses to these three species has been problematic. Classification based on the animal host species has proved unsatisfactory because pestiviruses may cross species barriers. Classification systems based on serology have been confounded both by cross-reactivity between pestivirus species and antigenic variability within pestivirus species. In our laboratory we have used phylogenetic analysis, based upon comparison of highly conserved sequences in the 5' UTR, to segregate pestiviruses. Using this analysis we have found that all viruses of the BDV species belong to the same genotype. Similarly all viruses of the HCV species can all be segregated to the same genotype. In contrast, the BVDV species is made up of viruses that belong to two different genotypes. Characterization and recognition of differences between genotypes will lead to better reagents for the detection and control of pestivirus infections.

Last Modified: 11/28/2014
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