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ARS Home » Research » Publications at this Location » Publication #74983
Title: MAINTENANCE OF A COMPETITIVE EXCLUSION CULTURE BY CONTINUOUS-FLOW FERMENTATION

Author
item Nisbet, David
item Corrier, Donald
item RICKE, STEVEN - TEXAS A&M UNIVERSITY
item Hume, Michael
item DELOACH, JOHN - 6202-30-10

Submitted to: Western Poultry Disease Conference
Publication Type: Proceedings
Publication Acceptance Date: 9/27/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: A competitive exclusion (CE) culture was isolated by the continuous flow (CF) culture of chicken cecal bacteria. The original CF culture (CF3) and a CF culture derived from a lyophilized sample of CF3 (LCF3) that had been stored for 180 days at -70C were provided to day old chicks. Experiments utilizing CF3 were performed after 5, 30, 100 and 365 days of CF conditions, and LCF3 after 5 days of CF culture. In all experiments, chicks were challenged with 10**4 CFU of Salmonella typhimurium at 3 days of age. At 10 days of age, chicks were killed and cecal contents analyzed for fermentation parameters and for S. typhumurium colonization. Ten day old chicks provided CF3 or LCF3 had increased cecal propionic acid (P<0.05) and decreased Salmonella cecal colonization (P<0.05) compared to untreated controls in all experiments. Results indicate that CF cultures can be used as a practical method to maintain CE cultures without loss of efficacy, and CF cultures stored in a lyophilized form can be reconstituted and still maintain efficacy.