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United States Department of Agriculture

Agricultural Research Service

Title: Simple Sequence Repeat (Ssr) Marker Development and Progress Towards the Integration of Soybean Molecular Genetic Maps

Authors
item Cregan, Perry
item Lark, Karl - UNIVERSITY OF UTAH
item Shoemaker, Randy
item Specht, Jim - UNIVERSITY OF NEBRASKA
item Kahler, Alex - BIOGENETIC SERVICES, INC.

Submitted to: Biennial Conference on Molecular and Cellular Biology of the Soybean
Publication Type: Abstract Only
Publication Acceptance Date: September 29, 1996
Publication Date: N/A

Technical Abstract: A collaborative effort is proceeding with the goal of developing 500 or more Simple Sequence Repeat (SSR) DNA markers in soybean. The level of informativeness of each new marker locus assessed on a set of 10 soybean cultivars to assure a maximum level of genetic polymorphism of each marker. The average gene diversity of newly developed markers during the e past 3 month period was 0.62 when assayed on these 10 soybean cultivars. As markers are developed each is mapped in three different populations including the USDA/ISU F2 mapping population derived from a cross of Glycine max (A81-356022) x G. soja (PI 468.916), a second F2 population derived from a cross of the cultivars Clark x Harosoy, and a set of recombinant inbred lines from Minsoy x Noir 1. A set of selected SSR markers were used to characterize each of the mapping populations. At least two markers have been placed in each of 17 of the 24 linkage groups s previously defined in the G. max x G. soja population and commonly referenced in soybean molecular genetic mapping research. These include linkage groups a2, b1, b2, c2, d1, d2, e, f, g, h, i, j, k, l, n, p, and q. By the end of 1996 we hope to detect homologies between all linkage groups of the three mapping. This will allow the complete joining of the three maps and provide a standard set of reference loci for scientists engaged in the development or use of molecular genetic maps of the soybean genome.

Last Modified: 8/21/2014
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