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United States Department of Agriculture

Agricultural Research Service

Title: Utilization of Isozyme Markers for Evaluation of Tropical Fruit and Nut Germplasm at the Usda/ars Clonal Germplasm Repository

Authors
item Zee, Francis
item Aradhya, Malli - UNIVERSITY OF HAWAII
item Manshardt, Richard - UNIVERSITY OF HAWAII
item Sui, Li - UNIVERSITY OF HAWAII

Submitted to: Agricultural Research International Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: June 23, 1996
Publication Date: N/A

Technical Abstract: In facing the current fiscal restraints and limitations in land availability and manpower for germplasm preservation, it is critical for the repository to streamline and be able to do more with less. The process of verification of authenticity of collection, elimination of redundancy, and expansion of diversity can be enhanced. Enzyme electrophoresis is a simple, inexpensive and less time consuming tool for evaluation of germplasm, especially in tree crops which are clonally propagated. Isozyme fingerprinting helps in establishing the genetic identity of clonal material, in assessing the genetic diversity and classification of germplasm, and in patenting of clonal cultivars. Although the information presented was generated from a relatively small collection, especially the wild species and with limited passport information, this is an attempt to identify the strengths and weaknesses of the current collection, and to develop strategies for future collection.

Last Modified: 11/23/2014
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