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United States Department of Agriculture

Agricultural Research Service

Title: Supercritical Fluid Methods for Lipid Analysis and Extraction

Authors
item Snyder, Janet
item Taylor, Scott
item Eller, Fred
item King, Jerry

Submitted to: Pittsburgh Conference
Publication Type: Abstract Only
Publication Acceptance Date: March 21, 1997
Publication Date: N/A

Technical Abstract: Supercritical fluid chromatography (SFC) as a practical analysis method for lipid mixtures have been developed using a SB-Octyl-50 capillary column and flame ionization. Samples analyzed by capillary SFC include reaction products from lipase-catalyzed methanolysis of triglycerides to fatty acid methyl esters, analysis of nutrients from food products, tocopherol analysis of Vitamin E-enriched samples, glycerolysis reaction products, and randomized lard compositions. Methods utilizing SFC with a silica-packed column and evaporative light scattering detection (ELSD) were developed using modifiers and additives to analyze the more polar lipids, such as, phospholipids. Although SFC is an effective technique for the rapid analyses of complex lipid containing mixtures, with minimal sample preparation, supercritical fluid extraction (SFE) can be employed along with SFC advantageously. SFE of lipid mixtures has been developed for the concentration of specific lipid moieties and the isolation of lipids from varied matrices, such as cholesterol from food products prior to SFC analysis. Extraction of lipid matter can be matrix dependent as with cereal-based samples and requires the use of cosolvents and hydrolyzing agents to extract intractably-bound lipid species. Results from these studies and the complementary role of SFC in confirming extraction and conversion efficacy will conclude the presentation.

Last Modified: 4/21/2014
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