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United States Department of Agriculture

Agricultural Research Service

Title: Use of a Dot-Blot Assay to Monitor Serologic Responses in Brucella Abortus Strain Rb51-Vaccinated Cattle

item Olsen, Steven
item Stevens, Mark
item Cheville, Norman - IA STATE UNIVERSITY

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: November 12, 1996
Publication Date: N/A

Technical Abstract: The purpose of this study was to evaluate a dot-blot assay using (gamma)-irradiated Brucella abortus strain RB51 (SRB51) bacteria for its specificity and sensitivity to detect antibody responses of SRB51-vaccinated cattle. Dot-blot titers of cattle vaccinated with approximately 1 x 10(10) CFU of SRB51 were similar to those of cattle vaccinated with similar numbers of B. abortus strain 19 (S19), and greater (P<0.05) than titers of non-vaccinated cattle. In the first 12 weeks after vaccination, the dot-blot assay had a 100% specificity for titers of 80 or less and a 53% sensitivity for titers of 160 or greater. Sensitivity of the dot-blot assay peaked at 4 weeks after vaccination. Dot-blot responses of cattle vaccinated with 1 x 10(9) CFU of SRB51 did not differ (P>0.05) from titers of non-vaccinated cattle. Following intraconjunctival challenge with B. abortus strain 2308, titers on the dot-blot assay did not tdiffer (P>0.05) between S19-, SRB51-, and non-vaccinated cattle. Data fro this study suggests that this assay will be of benefit for monitoring immune responses of cattle following SRB51 vaccination.

Last Modified: 4/22/2015
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