Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: May 17, 1996
Publication Date: N/A
Technical Abstract: Hog cholera, bovine viral diarrhea, and border disease viruses (HCV, BVDV, and BDV, respectively) are antigenically and genetically closely related pestiviruses. The accurate, rapid differential diagnosis of HCV and BVDV and BDV infections in swine is vital in detecting the possible reintroduction of HCV into national herds from which the virus has been eradicated. A nested polymerase chain reaction (PCR) amplification system for each of two pestiviral genomic segments is described. Amplification of the relatively conserved 5' genomic terminus identified 59 of 61 HCV, BVDV, and BDV generically as pestiviruses. Nested amplification of the second region was designed to differentiate HCV from BVDV and BDV by exploiting relatively conserved differences in the nucleotide sequences encoding the major envelope glycoprotein. This second PCR correctly identified 36 of 36 diverse HCV isolates while failing to recognize any of 25 BVDV and BDV. Multiple restriction fragment length analyses confirmed the identities of both external and internal (nested) PCR products. The two sets of PCR reactions, when used together, help confirm the generic identification of most pestiviruses while simultaneously permitting the crucial diagnostic differentiation of HCV from BVDV and BDV.