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United States Department of Agriculture

Agricultural Research Service

Title: Analyses of Monoclonal Antibodies Reacting with Porcine Wcd6: Results of the Second International Swine Cd Workshop

Authors
item Pescovitz, M - INDIANA UNIVERSITY
item Book, B - INDIANA UNIVERSITY
item Aasted, B - ROYAL VET & AGRI UNIV
item Dominguez, J - INIA
item Bullido, R - INIA
item Trebichavsky, I - INST MICROBIOLOGY PRAGUE
item Novikov, B - ALL-RUSSIAN RIVVM
item Valpotic, I - UNIVERSITY OF ZAGREB
item Lunney, Joan
item Boyd, Patricia

Submitted to: Veterinary Immunology and Immunopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: October 12, 1997
Publication Date: N/A

Interpretive Summary: During the Second International Swine CD Workshop only one new anti-CD6 monoclonal antibody (mAb) was identified. This mAb, MIL8, reacted similarly to other CD6 mAb; thus, it differentiates the subset of T lymphocytes which is important in killing virus infected cells from the T cell subset that acts as a natural killer cell, killing tumor cell lines. Production of an mAb which recognizes a new determinant on this CD6 molecule will help scientists target this important cell subpopulation in their detailed immune interaction studies. The international workshop, sponsored by the International Union of Immunological Scientists, enables scientists worldwide to independently verify the reactivity of panels of mAb and thus to precisely identify important new reagents to aid analyses of swine immune responses, particularly those involved in controlling disease and vaccine responses.

Technical Abstract: Among the 57 monoclonal antibodies analyzed within the T-cell group of the Second International Swine CD Workshop, one mAb fell within cluster T14a that included the CD6 standard a38b2 (No. 175). The new mAb MIL8 (No. 082) and a38b2 both precipitated a 150 kDa monomeric protein. Staining patterns on the various cell types were similar. There was no inhibition of binding of either mAb to peripheral blood T-cells with the opposite mAb. The new mAb, MIL8, therefore reacts with a separate epitope, from wCD6a. The epitope with which MIL8 reacts is assigned as wCD6b.

Last Modified: 10/21/2014
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