Submitted to: Mammalian Genome
Publication Type: Abstract Only
Publication Acceptance Date: November 9, 1997
Publication Date: N/A
Technical Abstract: Five chemokine genes, transforming growth factors alpha, beta 2 and 3 (TGFBA, TGFB-2 and TGFB-3), interleukin 8 (IL-8) and monocyte chemoattractant protein 2 (MCP-2), were mapped to porcine linkage groups on chromosomes 3q, 10p, 7q, 8 and 12q, respectively. Restriction fragment length polymorphisms (RFLP) for these genes were developed by Southern blot hybridization after digestion of porcine genomic DNA with BamHI and MspI (TGFBA), BamHI and PvuII (TGFB-2), HindIII (TGFB-3), BglII (IL-8) and PstI (MCP-2) and used to genotype the USDA-MARC Swine Reference Population. Sufficient informative meiosis for TGFBA (22), TGFB-2 (38), TGFB-3 (28), IL-8 (58), and MCP-2 (156), were available to establish initial linkage (LOD > 3) by two-point pairwise linkage analysis with over 1,000 existing loci in the USDA-MARC genome database. Multi-point analysis using CRIMAP determined the most likely order for each new marker. The assignment of the five chemokine genes in swine concurs with previous porcine/human chromosomal homologies based on results from ZOO-FISH and chromosomal painting experiments.