Page Banner

United States Department of Agriculture

Agricultural Research Service

Title: Transcriptional Expression of a Putative Tachykinin-Like Peptide Receptor Gene from Stable Fly

Author
item Guerrero, Felix

Submitted to: Peptides
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: October 19, 1996
Publication Date: N/A

Interpretive Summary: STKR is a 4118 bp clone from a stable fly, Stomoxys calcitrans, cDNA library which encodes a protein with significant amino acid similarity to tachykinin-like peptide receptors in other organisms. Tachykinins and their receptors are involved in neuromuscular and sensory functions in both vertebrates and invertebrates. PCR was utilized to examine the receptor gene expression from various life stages of the stable fly and determine i which stage the mRNA was most abundant. STKR expression was significant in pupae and adults, particularly in isolated adult fly gut. Expression in development embryos was low but detectable, while larval expression was intermediate between early embryos and pupae.

Technical Abstract: STKR is a 4118 bp clone from a stable fly, Stomoxys calcitrans, cDNA library which encodes a protein with significant amino acid identity to tachykinin-like peptide receptors. RT-PCR was utilized to examine the transcriptional expression from various life stages of the stable fly. STKR expression was significant in pupae and adults, particularly in isolated adult fly gut. Expression in developing embryos was low but detectable, while larval expression was intermediate between early embryos and pupae.

Last Modified: 8/22/2014
Footer Content Back to Top of Page